Table II.

Changed proteins identified by ESI-FTICR-MS

The spectrum for each ICAT-labeled peptide is illustrated in supplemental Fig. S2. Changed proteins that were identified by both MALDI-TOF MS/MS and FT-MS are bold.

No.	Swiss-Prot ID	Protein name	Molecular weight	Scorea	Pep.b	Ratio		Loc.e	Function
						1mc	6md
1	OAT_MOUSE	Ornithine aminotransferase	48,324	14	1	1.55	2.3	MC	Transaminase
2	GLNA_MOUSE	Glutamine synthetase	42,092	100	2	1.82	2.1	MC	Glutamine biosynthesis
3	AL4A1_MOUSE	Δ1-Pyrroline-5-carboxylate dehydrogenase	61,772	38	2	1.5	1.03	MC	l-Glutamate biosynthesis
4	URIC_MOUSE	Uricase	35,017	65	2	0.88	0.43	MC	Oxidation of uric acid
5	ACOX1_MOUSE	Acyl-coenzyme A oxidase 1	74,587	146	3	1.02	0.64	MC and Pero	Lipid oxidation
6	ECHP_MOUSE	Enoyl-CoA hydratase	78,194	132	4	1.05	0.57	MC and Pero	Lipid oxidation
7	HMCS2_MOUSE	HMG-CoA synthase	56,786	109	3	1.15	0.65	MC	Ketone body biosynthesis
8	NDUS1_MOUSE	NADH-ubiquinone oxidoreductase 75-kDa subunit	79,698	39	1	0.72	0.5	MC	Respiratory chain
9	NDUB7_MOUSE	NADH-ubiquinone oxidoreductase B18 subunit	16,320	62	1	0.95	0.63	MC	Respiratory chain
10	NDUV1_MOUSE	NADH-ubiquinone oxidoreductase 51-kDa subunit	50,802	50	2	0.89	0.62	MC	Respiratory chain
11	BHMT1_MOUSE	Betaine-homocysteine S-methyltransferase 1	44,992	236	4	2.2	1.05	Cyto	Methionine biosynthesis
12	CAH3_MOUSE	Carbonic anhydrase 3	29,348	21	1	0.31	0.32	Cyto	Hydration of carbon dioxide
13	HYEP_MOUSE	Epoxide hydrolase 1	52,542	53	1	1.0	0.58	ER	Epoxide hydrolase

^aMolecular weight search (MOWSE) scores obtained by the search of MS/MS data using Mascot engine.

^bNumbers of peptides identified by FT-MS.

^cProtein expression ratios of SAMP8/SAMR1 at 1 month old.

^dProtein expression ratios of SAMP8/SAMR1 at 6 months old.

^eProtein subcellular locations. MC represents mitochondria, Pero represents peroxisome, Cyto represents cytoplasm, and ER represents endoplasmic reticulum.