Table I.
Hyperphosphorylation of 13 proteins in PTP1B-deficient fibroblasts under basal cell culture conditions
wt, wild type; pY, phosphotyrosine; pS, phosphoserine; pT, phosphothreonine; (0.5), 50% phosphorylation probability.
| Function | Protein | Phosphopeptide sequence | Tyr(P) peptide
|
Whole protein KO/wt ratio | |||
|---|---|---|---|---|---|---|---|
| Tyr(P) site | p value | KO/wt ratio | Normalized | ||||
| Cell shape and motility | LPP | SAQPSPHYMAGPSSGQIpYGPGPR | 245 | 0.05 | 4.09 | 2.62 | 1.56 |
| pY(0.5)pY(0.5)EPYpYAAGPSYGGR | 297/302 | 0.05 | 4.02 | 2.58 | 1.56 | ||
| SEGDTApYGQQVQPNTWK | 318 | 0.05 | 3.44 | 2.21 | 1.56 | ||
| EAApYAPPASGNQNHPGMYPVSGPKK | 333 | 0.04 | 4.26 | 2.73 | 1.56 | ||
| MLpYDMENPPADDYFGR | 403 | 0.01 | 6.96 | 4.47 | 1.56 | ||
| Cell motility | Vav-3 | TPIALATGIRPFPTEESINDEDIpYK | 141 | 0.00 | >10.00 | ||
| Tks5/Fish protein | VKYEEPEpYDVPAFGFDpSEPEMNEEPSGDR | 557 | 0.08 | 3.42 | |||
| VGESSEDVALEEETIpYENEGFRPYTEDTLSAR | 619 | 0.02 | 5.21 | ||||
| Fer | VQENDGKEPPPVVNpYEEDAR | 402 | 0.03 | 4.70 | 3.45 | 1.36 | |
| QEDGGVpYSSSGLK | 715 | 0.04 | 4.47 | 3.29 | 1.36 | ||
| RhoGAP12 | ApT(0.5)pT(0.5)PPNQGRPDpSPVpYANLQELK | 241 | 0.08 | 3.32 | |||
| Cell motility/vesicular transport | Cortactin | NASTFEEVVQVPSApYQK | 334 | 0.02 | 5.58 | 5.44 | 1.03 |
| KQpTPPApSPpSPQPIEDRPPpS(0.5)pS(0.5)PI pYEDAAPFK | 421 | 0.03 | 4.86 | 4.74 | 1.03 | ||
| Cell adhesion | Catenin δ-2 | DpYETYQPFPNSTR | 1176 | 0.00 | 7.73 | ||
| p120ctn | QDVpYGPQPQVR | 257 | 0.06 | 3.84 | 4.13 | 0.93 | |
| FHPEPpYGLEDDQR | 280 | 0.10 | 3.07 | 3.30 | 0.93 | ||
| Tight junction assembly | ZO-1 | TSTLRHEEQPAPApYEVHNR | 1164 | 0.06 | 3.72 | 3.71 | 1.00 |
| YRPEAQPpYSSTGPK | 1177 | 0.00 | >10.00 | >10.00 | 1.00 | ||
| IP3 and DAG signaling | PLCγ1 | IGTAEPDpYGALYEGR | 771 | 0.16 | 2.50 | 2.54 | 0.98 |
| Phagocytosis | MEGF10 protein | DSPpYAEINNSTPANR | 1061 | 0.00 | >10.00 | ||
| Proliferation | p62DOK | FSALEMLENSLpYSPTWEGSQFWVTSQK | 146 | 0.16 | 2.45 | 2.03 | 1.21 |
| GLpYDLPQEPR | 376 | 0.25 | 1.87 | 1.54 | 1.21 | ||
| Proliferation/migration | Eph receptor A3/4/5 | VLEDDPEAApYTTR | 779 | 0.06 | 3.85 | ||
| Cell growth/metabolism | Insulin-like growth factor 1 receptor/insulin receptor | DIpYETDpYYRK | 1193/1197 | 0.00 | 8.75 | ||
| Unknown | Similar to oligophrenin 1 | LWLEAMDGKEPIpYTLPAIISK | 371 | 0.06 | 3.70 | ||