Table II.

Alterations in EGF signaling in PTP1B-deficient cells

EGF stimulation led to increased phosphorylation of three proteins in KO cells compared with wild-type cells. wt, wild type; pY, phosphotyrosine.

Function	Name	Phosphopeptide sequence	Tyr(P) peptide				Whole protein KO/wt ratio	Stimulation ratio EGF/basal
			Tyr(P) site	p value	KO/wt ratio	Normalized
Cell motility	Vav-3	TPIALATGIRPFPTEESINDEDIpYK	141	0.00	10.58			23.60
IP3 and DAG signaling	PLCγ1	IGTAEPDpYGALYEGR	771	0.04	5.94	6.05	0.98	12.87
Phosphoinositide signaling	SHIP2	TLSEVDpYAPGPGR	1136	0.10	4.06	6.22	0.65	48.61
Proliferation	p62DOK	GFSSDTALpYSQVQK	450	0.18	3.04	2.51	1.21	2.38
Proliferation/migration	Epidermal growth factor receptor	pYSSDPTGAVTEDNIDDAFLPVPEpYVNQSVPK	1069/1092	0.39	1.31	1.68	0.78	45.04
		MHLPSPTDSNFpYR	1000	0.39	1.20	1.55	0.78	>50.00
		GSHQMSLDNPDpYQQDFFPK	1172	0.38	1.05	1.35	0.78	37.97
		GPTAENAEpYLR	1197	0.37	1.02	1.32	0.78	53.28