Table III.

Alterations in PDGF signaling in PTP1B-deficient cells

PDGF stimulation led to hyperphosphorylation of five proteins in PTP1B KO cells. wt, wild type; pY, phosphotyrosine; (0.5), 50% phosphorylation probability.

Function	Name	Phosphopeptide sequence	Tyr(P) peptide				Whole protein KO/wt ratio	Stimulation ratio PDGF/basal
			Tyr(P) site	p value	KO/wt ratio	Normalized
Phagocytosis	MEGF10 protein	DSPpYAEINNSTPANR	1061	0.01	>10.00			2.14
IP3 and DAG signaling	PLCγ1	IGTAEPDpYGALpYEGRNPGFpYVEANPMPTFK	771/775/783	0.05	6.11	6.22	0.98	28.47
		IGTAEPDpYGALYEGR	771	0.14	3.96	4.04	0.98	23.29
Proliferation/migration	PKCδ	KLDTTESVGIpYQGFEK	311	0.03	7.23	7.96	0.91
Ras positive regulation	SHP2	IQNTGDpY(0.5)pY(0.5)DLYGGEK	62 or 63	0.09	4.77	3.85	1.24	>20.00
Ras negative regulation	p120RasGAP	EIpYNTIR	451	0.04	6.66	4.42	1.51
Proliferation	p62DOK	IPPGPSQDSVpYSDPLGSTPAGAGEGVHSK	314	0.24	2.92	2.41	1.21
		TVPPPVPQDPLGSPPALpYAEPLDSLR	295	0.27	2.68	2.22	1.21
Proliferation/migration	Platelet-derived growth factor receptor β	YADIESPSYMAPYDNpYVPSAPER	778	0.27	2.63	1.53	1.72	18.21
		DESIDpYVPMLDMK	751	0.31	2.38	1.39	1.72	>50.00
		DIMRDSNpYISK	857	0.33	2.18	1.27	1.72	21.75
		pYQQVDEEFLR	970	0.36	2.00	1.16	1.72	73.45