Table 1.
Protein | Primer (5′–3′) | Probe (5′–3′) | Product size (bp) | |
---|---|---|---|---|
β-Actin | S* | TCTGGCTCCTAGCACCATGA | ATCAAGATCATTGCTCCTCCTGAGCGC | 72 |
AS* | CCACCGATCCACACAGAGTACT | |||
IFN-γ | S | AGCAACAGCAAGGCGAAA | TCAAACTTGGCAATACTCATGAATGCATCCT | 72 |
AS | CTGGACCTGTGGGTTGTTGA | |||
IL-1β | S | CTCATTGTGGCTGTGGAGAA | TGGCAGCTACCTGTGTCTTTCCCG | 78 |
AS | GGTGCTCATGTCCTCATCCT | |||
IL-6 | S | CCCAATTTCCAATGCTCTC | TAGCCACTCCTTCTGTGACTCCAGCT | 77 |
AS | TGAATTGGATGGTCTTGGTC | |||
IL-10 | S | CAGCCGGGAAGACAATAACTG | CCCACTTCCCAGTCGGCCAG | 67 |
AS | CCGCAGCTCTAGGAGCATG | |||
IL-12p40 | S | AAGTGTGAAGCACCAAATTACTC | ACGGTTCACGTGCTCATGGCT | 71 |
AS | TTCAAGTCCATGTTTCTTTGC | |||
MIP-1α | S | ACTAAGAGAAACCGGCAGAT | TGCGCTGACTCCAAAGAGACC | 77 |
AS | TTCAGTTCCAGGTCAGTGAT | |||
TNF-α | S | CCGATGGGTTGTACCTTGTCT | TCTTCAAGGGACAAGGCTGCCCC | 76 |
AS | TGGGTGAGGAGCACGTAGTC |
S, sense; AS, antisense.