. Author manuscript; available in PMC: 2008 Sep 29.

Published in final edited form as: Biochemistry. 2006 Dec 5;45(48):14371–14378. doi: 10.1021/bi061662p

Table 2.

Steady-State Parameters for the V5C/K79C Covalent Complex at Various Ionic Strengths^a

Ionic Strength (mM)	k_bi (μM⁻¹s⁻¹)	K_M(μM)	k_cat (s⁻¹)
10	4.6 ± 0.4	250 ± 80	1100 ± 300
20	3.2 ± 0.2	120 ± 20	390 ± 40
30	3.4 ± 0.3	210 ± 80	740 ± 220
50	6.4 ± 1.2	56 ± 19	360 ± 60
70	53 ± 8	4.6 ± 0.8	240 ± 10
100	150 ± 10	2.7 ± 0.3	410 ± 10

Substrate: recombinant yeast iso-1 ferrocytochrome c(C102T), [H₂O₂] = 200 μM, potassium phosphate buffer, pH 7.5, 25 °C.