Table 1.
Kinetic parameters with Fhit
| Compound |
Kcat1 (s-1) |
Km1 (μM) |
Kcat /Km1 (s-1 M-1) |
Kcat /Km2 (S-1 M-1) |
Km3 (μM) |
Kr3 (μM) |
|---|---|---|---|---|---|---|
| ApppA | 73 ±4.3 | 1.9 ±0.2 | 3.8 E7 | 2.0 ±0.2 | ||
| ApppAMC | 1.2 E6 | |||||
| ApppBODIPY | 7.3 | 3.0 | 2.4 E6 | 2.3 E6 | ||
| GpppBODIPY | 0.58 | 1.4 | 4.1 E5 | 5.8 E5 | ||
| AppppA | 2.6 ±0.4 | |||||
| ApppppA | 13.9 ±2.3 | |||||
| PPi | 19.9 ±3.8 | |||||
| AMP | 196 ±25 | |||||
| ATP-αS | 163 ±19 | |||||
| GTP-αS | 257 ±34 | |||||
| Pi | 2900 ±430 |
1
From initial rate assays. ApppA data are from Pace et al. (1998).
2
From substrate decay assays.
3
By titration into ApppBODIPY decay assays.