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. 2008 Sep 23;4(6):338–344. doi: 10.7150/ijbs.4.338

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Northern analysis of E. coli genes that appear to be regulated by LexA. RNA was extracted from three isogenic strains that differed in lexA gene: RW118 (lexA⁺), RW434 (lexA[Ind^-]), and RW542 (lexA51[Def]). RNA was obtained from undamaged cells (-) and from cells that had been exposed to mitomycin C (5 µg ml-1) (+) for 30 min before extraction. The previously identified LexA-regulated recA and umuDC genes were used as positive controls. The genes are depicted according to their ascending heterology index (HI). The sizes of the mRNA transcript and the possible functions of the genes are also indicated See ref. 28 for more details. (By courtesy of Blackwell Science).