Fig. 2.

Both P2 and P18 NP proteins inhibit IFN-β expression. 293T cells were transfected with an IFNβ-LUC reporter plasmid, together with either empty vector or vector expressing P2 NP, P18 NP, Lassa virus NP, or influenza A virus NS1. A β-gal expression plasmid was included in each reaction for normalization purpose. Cells were infected with or without Sendai virus to induce IFN-β expression. Luciferase activity was measured at 24 hpi and normalized against β-gal activity.