Figure 4.

(A–D) Electron microscopic analysis of WT and ΔKIN1ΔKIN2 cells. (A) Longitudinal section through WT axoneme. (B) Longitudinal section through axonemal remnant of a ΔKIN1ΔKIN2 cell. (C and D) Cross-sections through WT and double knockout basal bodies, respectively. Cells were processed for thin-section electron microscopy 84 h after pair isolation. (E) Analysis of the length of ciliary axonemes in ΔKIN1ΔKIN2 and WT cells, measured on confocal sections of cells labeled for tubulin by the SG serum using the NIH Image software package. Values are mean ± SD (n = 110 and 120 for wild-type and ΔKIN1ΔKIN2 cells, respectively).