Table 1.
Strains used in this study
| Strain | Micronuclear genotype | Macronuclear genotype | Macronuclear phenotype (known drug resistances, mating types) |
|---|---|---|---|
| B2086.1 | WT | WT | II |
| CU427.3 | chx1-1/chx1-1 | WT | cy-s, VI |
| CU428.1 | mpr1-1/mpr1-1 | WT | mp-s, VII |
| B*VII | nonfunctional mic (star) | WT | VII |
| UG1 | kin1∷neo2/kin1∷neo2 | kin1∷neo2 | pm-s |
| UG2 | kin1∷neo2/kin1∷neo2 | kin1∷neo2 | pm-s |
| UG3 | WT | WT | |
| UG4 | WT | WT | |
| UG7 | kin2∷bsr1/kin2∷bsr1 | kin2∷bsr1 | bs-r |
| UG8 | kin2∷bsr1/kin2∷bsr1 | kin2∷bsr1 | bs-r |
| UG9 | WT | WT | |
| UG10 | WT | WT | |
| UG11 | WT | kin2∷neo2 | pm-r |
| UG12 | WT | kin2∷neo2 | pm-r |
| UG13 | kin1∷neo2/kin1∷neo2, kin2∷bsr1/kin2∷bsr1, | KIN2/kin2∷bsr1 | bs-r, pm-s, mp-s |
| UG14 | kin1∷neo2/kin1∷neo2, kin2∷bsr1/kin2∷bsr1, mpr1-1/mpr1-1 | WT | bs-s, pm-s, mp-s |
Strains B2086.1, CU428.1, and CU427.3 were kindly provided by Dr. P. Bruns (Cornell University, Ithaca, NY). UG1 and UG2 strains are germ line transformation derivatives which have disruption of the KIN1 gene. Please note that although all copies of KIN1 in the MAC are disrupted with the neo2 gene, these cells are sensitive to pm because of apparent silencing of neo2 gene (our unpublished observations). However, when UG1 and UG2 are crossed, their progeny cells temporarily express dominant pm resistance allowing for genetic identification of the neo2 gene. The UG3 and UG4 strains are wild-type controls derived from the same parental strains that were used to construct UG1 and UG2. UG7 and UG8 are germ line transformation derivatives, which have disruption of the KIN2 gene. UG9 and UG10 strains are wild-type controls derived from the same parental strains (heterozygous for KIN2/kin2∷bsr1) that were used to construct UG7 and UG8.