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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Inorg Biochem. 2008 Jun 13;102(9):1825–1830. doi: 10.1016/j.jinorgbio.2008.06.003

Figure 4. EPR spectram of 1.6 mM ETHE1 under different conditions.

Figure 4

A, ETHE1 as-isolated containing 0.33 equivalents of Fe; B, iron-enriched ETHE1 containing 1.2 equivalents of Fe; C, iron-enriched ETHE1 after 2 cycles of freeze/thaw; D, iron-enriched ETHE1 after 4 days at 4º C. Spectra were collected on a Bruker ESP-300E spectrometer containing an ESR-900 helium flow cryostat operating at 4.7 K with 2 milliwatts of microwave power at 9.48 GHz and 10 G-field modulation at 100 kHz.