Figure 2.

Tlg2p is not glycosylated. FHER001-5A cells transformed with pHA-TLG2 were grown to midlogarithmic phase, and the protein extracts were prepared and incubated in the absence (−) or presence (+) of endoglycosidase H. Extracts were loaded on a 9% polyacrylamide/0.1% SDS gel and analyzed by Western immunoblotting. (A) Detection of HA-Tlg2p (arrowhead). The tagged protein was able to complement the endocytic defect of uracil permease. (B) Detection of CPY. The position of mature CPY (m) or deglycosylated CPY (d) is indicated to the right. Each lane contains proteins extracted from ∼6 × 10⁶ cells. Numbers to the left of each panel indicate the position of molecular weight markers loaded onto the same gel.