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Accumulation of vesicles in tlg2Δ cells. Wild-type and tlg2Δ cells were grown to midlogarithmic phase in YPD medium at 30°C. Intact cells were fixed with glutaraldehyde, stained with KMnO4, embedded, and sectioned as described by Goodson et al. (1996). (A) The numbers of vesicles per cell section were counted in 100 cells. (B) A typical tlg2Δ cell with accumulated vesicles, indicated by the arrows. N, Nucleus; V, vacuoles. Bar, 1 μm.
