Figure 7.

Internalization of positively charged Nanogold. Wild-type (A and B) and tlg2Δ (C–I) spheroplasts were incubated with positively charged Nanogold on ice for 5 min and then shifted to room temperature for 15 min. The cells were fixed, dehydrated, and embedded. Thin sections were generated and enhanced with HQ Silver and visualized in the electron microscope. Endocytic vesicles found in tlg2Δ cells are labeled with arrows; an early endosome from wild-type cells is labeled with an asterisk, as is a structure from tlg2Δ cells, which resembles an early endosome somewhat. The triangle in A indicates late endosomes. Bar, 200 nm.