Table 1. Characterization of polyplex internalization.a.
| Polymer Usedb | Percent Internalization of Polyplexesc | Geometric Mean Fluorescenced |
|---|---|---|
| 1a | 1.89% (±0.06%) | 46.5 (±0.9) |
| 1b | 1.46% (±0.06%) | 45.0 (±1.9) |
| 1c | 21.53% (±0.89%) | 49.8 (±0.4) |
| 1d | 96.13% (±1.16%) | 452.7 (±5.3) |
| 1e | 92.88% (±0.43%) | 219.7 (±1.0) |
| LPEI | 90.42% (±1.30%) | 225.7 (±2.3) |
a
Based on flow cytometry experiments using COS-7 cells and polyplexes formed using Cy-5 labeled DNA (see main text and Materials and Methods for additional experimental details).
b
Polyplexes were prepared at polymer/DNA ratios (w/w) identical to those shown in Figure 1, and correspond to those ratios determined to mediate the highest levels of transfection for each polymer (1:1 for polymers 1a, 1c, 1e, and LPEI; 1:9 for polymer 1b; 1:7 for polymer 1d.)
c
Values are expressed as the percent of Cy5-positive cells relative to all cells interrogated.
d
Geometric mean fluorescence intensity of Cy5-positive cells gated against negative controls.