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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Biomol Screen. 2008 Jul 14;13(7):591–608. doi: 10.1177/1087057108321089

Figure 1.

Figure 1

HTS-assay set-up and evaluation. Cells seeded in a 384-well plate format were infected with wild-type MV strain Alaska at an MOI of 0.2 when reaching a density of 3,000/well. Prior to infection, wells were treated with the MV RdRp inhibitor AS-136A (final concentration 5 μM) or an equal volume of solvent (DMSO) as indicated. Mock-infected wells received equal volumes of media and DMSO, and media control wells (w/o) lacked target cells. Plates were fixed and stained with crystal violet 72 hours post-infection and absorbance at 560 nm determined. Two representative wells each are shown, numbers below the wells reflect averages ± SD of 16 replicates.