Figure 3.
Quantitative assessment of entry inhibitor candidates using a firefly luciferase reporter-based cell-to-cell fusion assay. Effector cells expressing T7 polymerase and plasmid-encoded MV H and F envelope glycoproteins were overlaid in the presence of different candidate compound concentrations with target cells harboring the luciferase reporter construct under the control of the T7 promoter. Luciferase activities in cell lysates were determined as an indicator for the extend of cell-to-cell fusion five hours post-overlay. Graphs represent averages of four independent experiments, EC50 concentrations are shown.