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Contrast enhancement correlates with number of Gd-DOTAs bound. Solutions of mal-BSA (Gd-DOTA)n with n = 10 (bottom row) or 18 (top row) in distilled, deionized water, pH 7.0 were imaged at 7T at ambient temperature (25°C). Signal intensity increases as concentration increases (from right to left) and greater signal intensity is observed for the more heavily labeled agent. Contrast enhancement is observed for concentrations greater than 25-50μM.
