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. Author manuscript; available in PMC: 2008 Sep 29.
Published in final edited form as: Bioconjug Chem. 2006;17(2):538–547. doi: 10.1021/bc060018k

Figure 5.

Figure 5

Uptake of mal-BSA (Gd-DOTA)n is receptor-mediated. P388D1 cells were incubated with a fixed concentration of mal-BSA (Gd-DOTA)15 and increasing molar excesses of unlabeled mal-BSA. Representative samples are shown with molar excesses of competitor as indicated above each sample. Actual T1 values for the image appear below each sample, as well as average and standard deviation T1 for triplicate repeats. All values are given in seconds. Cells were imaged at 7T. Increasing molar excess of unlabeled agent inhibits uptake of the labeled agent and reduced signal in the image. T1 decreases dramatically with molar excess of competitor. Competition by unlabeled ligand confirms that uptake of the contrast agent is receptor-mediated.