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. Author manuscript; available in PMC: 2008 Sep 29.
Published in final edited form as: J Med Chem. 2007 Jun 27;50(15):3423–3426. doi: 10.1021/jm070437i

Figure 4.

Figure 4

Surface plasmon resonance (SPR) data. The sensorgram of the binding of free DAQ B1 to immobilized human erythrocyte GAPDH. Analysis was conducted at concentrations from 40 μM to 2.5 μM. Binding parameters determined from fitting these data to a 2-state binding model are: kA 2.18 × 104 M−1 · s−1; kD 1.57 × 10−2 s−1; KD 7.2 μM. DAQ B1 and DAQ-biotin both behave in a manner characteristic of dose-dependent binding to GAPDH with a high level of specificity. Non-specific binding often shows SPR responses greater than theoretical, but the observed responses were less than the maximum predicted, which is explained by the expected loss of active GAPDH upon immobilization.