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. Author manuscript; available in PMC: 2008 Sep 29.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Aug;49(8):3677–3686. doi: 10.1167/iovs.07-1071

Figure 4.

Figure 4

Presynaptic and postsynaptic terminal labeling in Wt and Rs1-KO retinas during postnatal development. PSD95 (red) labels the photoreceptor synaptic terminal; calbindin (green) labels horizontal cell; PKCα (green) labels rod bipolar cell processes; mGluR6 (green) labels bipolar cell terminals. In Wt retina at P14 and P21, terminals of horizontal (A, D) and rod bipolar cell processes (B, E) colocalized with PSD95 immunoreactivity in the OPL. Punctate labeling of mGluR6 (green) and presynaptic PSD95 (red) were juxtaposed to each other at P14 (C) and P21 (F) in Wt retinas. Rs1-KO retinas at P14 showed similar staining for synaptic markers PSD95/calbindin (G), PSD95/PKCα (H), and mGluR6 (I). However, at P21 the regular arrangement of PSD95 in the OPL was disrupted in Rs1-KO retinas and horizontal (J) and rod bipolar cell (K) processes extended into the ONL. Aberrant extension of mGluR6 into the ONL was seen at P21 in conjunction with mislocalized presynaptic photoreceptor axons (L). mGluR6 and PSD95 were juxtaposed to each other at these punctate ectopic locations in the ONL. Scale bar, 25 μm