FIGURE 6. Identification of GalA in the dodecaprenyl phosphate-linked HexA isolated from R. leguminosarum.
Trimethylsilyl ester derivatives of methyl glycosides, obtained by methanolic HCl hydrolysis of the purified dodecaprenyl phosphate-linked HexA, were separated by gas-liquid chromatography. Peaks were identified by comparison of retention times with standards prepared in parallel. The chromatogram for the lipid donor was compared with those of GalA (see asterisks) and GlcA standards. No GlcA peaks are present in the natural product.