Table 3.

Summary of the affect of various mutations upon the pH effect in PI decay and the pH effect on the resting T2 Cu

	T1D	T1DE487D	T1DE487A	T1DD94E
Rate of PI decay pH = 5.0	1.8 × 10-3 s-1	1.6 ± 0.1 × 10-4 s-1	7.2 ± 0.5 × 10-5 s-1	3.7 ± 0.4 × 10-4 s-1
Rate of PI decay pH = 7.5	2.9 × 10-4 s-1	5.2 ± 0.9 × 10-5 s-1	8 ± 1 × 10-5 s-1	4 ± 1 × 10-5 s-1
Rate enhancement	6.2x	3.1x	1x	8.0x
KSIE @ low pH	0.89	2.0	~1	2.3
pH effect on resting T2 Cu	yes	yes	yes	No

The KSIE at high pH (measured at pH = 7.5) is 1.0 for T1D and all mutants