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. 1998 Oct;9(10):2891–2904. doi: 10.1091/mbc.9.10.2891

Figure 4.

Figure 4

RacC-induced morphology is inhibited by PI 3-kinase inactivation. (A and B) Control Ax2 cells; (C and D) RacC WT(+) cells. RacC WT(+) cells were also treated with the PI 3-kinase inhibitor wortmannin (2 μM) for 20 min (E and F). Cells were seeded on coverslips, pretreated with LY294002 or DMSO, fixed, permeabilized, stained with FITC–phalloidin, and viewed using phase-contrast (A, C, and E) or fluorescence (B, D, and F) microscopy. RacC WT(+) cells display the novel petalopodia actin-based structures on their surface (C and D; see arrowheads), whereas control Ax2 cells display actin in a cortical distribution (A and B). LY294002 treatment of RacC WT(+) cells reversed the formation of petalopodia (E and F) and caused the F-actin to redistribute to a more cortical pattern.