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Western blot analysis of the effect of LEK1 knockdown on actin and vimentin expression in DCs. WT and LEK1 knockdown DCs were pulsed with MoPn (MOI 1:5) and analyzed for actin and vimentin expression as described in the Materials and Methods section. Figure 4A = WT DCs; 4B = LEK-1 knock down DCs. Lane 1 = non-pulsed DC; Lanes 2, 3, 4, 5, 6, and 7 were MoPn-pulsed DC for 15′, 30′ 1h, 2h, 3h, and 4h respectively. Results are representative of 2 independent experiments.
