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. 2008 Oct 3;3(10):e3338. doi: 10.1371/journal.pone.0003338

Figure 1. Characterization of TPX2 during mouse oocyte meiotic maturation.

Figure 1

A: Mouse oocytes (n = 200) and Xenopus metaphase II oocyte lysate (equivalent to 1/4 of oocyte) were immunoblotted using an anti-TPX2 antibody. The upper band corresponds to TPX2. The lower band is non-specific [11]. B: TPX2 accumulates progressively during meiotic maturation. For each time point, 140 mouse oocytes were immunoblotted using anti-TPX2 (only the TPX2 specific band is presented) and anti-α-tubulin antibodies. C: Cdh1 controls TPX2 levels at GV (Prophase I). Western blot analysis of TPX2 and cdh1 in control oocytes and cdh1 morpholino injected oocytes (n = 100 for each group). D: Localization of TPX2 during meiotic maturation. Oocytes were double stained for TPX2 (green) and nucleic acids (blue) at the times indicated relative to GVBD. In meiosis I and II, TPX2 decorates the spindle microtubules. Scale bar is 10 µm.