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. 2008 Oct 3;3(10):e3338. doi: 10.1371/journal.pone.0003338

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Brunet et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Immunoblot analysis of TACC3 and phosphorylated TACC3 (P-TACC3) in GV and metaphase II (MII) oocytes. Oocytes (n = 150) were immunoblotted using an anti-TACC3 antibody (left) and an antibody raised against xTACC3 phosphorylated on Ser626 (right). B: Immunoblot analysis of TACC3 and P-TACC3 during meiotic maturation. Oocytes (n = 140) were immunoblotted at the indicated time points. C: Localization of P-TACC3 during meiotic maturation. Oocytes were triple stained for P-TACC3 (red), microtubules (green) and nucleic acids (blue). The P-TACC3 staining becomes restricted to the spindle poles during meiotic maturation. Scale bar is 10 µm.