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. 2008 Oct 8;3(10):e3369. doi: 10.1371/journal.pone.0003369

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Sanford et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Western blot analysis of cytoplasmic extracts prepared from cells transfected with pCGT7 of pCGT7 SF2/ASF (lanes 1 and 2, respectively). Blots were probed with anti-SF2/ASF and anti-GAPDH (left and right panel, respectively). B) Polyribosome association of endogenous mRNAs. Cytoplasmic extracts from control or pCGT7 SF2/ASF cells were resolved by sucrose gradient fractionation. The upper panel shows the absorbance of rRNA across the gradient, the positions of ribosomal subunits and complexes are indicated. Lower panels: RT-PCR analysis of RNA targets. Products were resolved by agarose gel electrophoresis. The sub-cellular fraction corresponding to the initial CLIP experiment is indicated below each gene symbol.