Figure 8. HIF-1α protein during murine RSV pneumonia in vivo.

Female, 6- to 8-week-old BALB/c mice were inoculated intranasally with purified RSV at 1×10⁷ plaque-forming units (PFUs), diluted in sterile 0.9 % sodium chloride for a total inoculation volume of 50 µl. As mock treatment, control mice were inoculated in the same way with an equivalent volume of sucrose diluted in 0.9 % sodium chloride. Lungs were removed at indicated time-points, and HIF-1α protein levels were determined by Western blot analysis (A) or quantified by densitometry, relative to beta-actin (B; *p<0.01).