Figure 10.

Quantification of the effects of caldesmon on the stimulation of focal adhesion formation by nocodazole and KCl treatment. Cells stained with anti-phosphotyrosine antibody and with anti-tag antibody visualizing transfected caldesmon were analyzed. The percentage of cells with small, medium, and large focal adhesions was scored. The diagram shows that transfection with caldesmon prevents stimulation of focal adhesion assembly by nocodazole, whereas stimulation by KCl is less severely affected.