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. 1999 Oct;10(10):3097–3112. doi: 10.1091/mbc.10.10.3097

Figure 4.

Figure 4

Caldesmon prevents development of traction forces in cells attached to an elastic silicone–rubber substrate. Cells were plated onto a fibronectin-coated silicone–rubber film 7 h after transfection with GFP-caldesmon (A–D), truncated caldesmon GFP–CD445B (E and F), or GFP alone (G and H) and photographed 20 h later. (A, C, E, and G) Phase-contrast images of substrate-attached cells and wrinkles they produce. (B, D, F, and H) GFP fluorescence images of the same fields showing that cells expressing full-length GFP–caldesmon cannot deform the substrate, whereas cells expressing truncated caldesmon, or GFP alone readily form wrinkles. Bar, 20 μm.