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Effect of caldesmon expression on the Rho-induced formation of focal contacts. Triple labeling was performed to visualize phosphotyrosine (A and D), VSV-tagged Rho A-V14 (B and E), and GFP–caldesmon in the same cells. (A–C) Cells cotransfected with activated Rho and full-length GFP-caldesmon show predominantly dot-like focal adhesions. (D–F) Cells cotransfected with activated Rho and truncated GFP–CD445B caldesmon form large and intensely stained focal adhesions. Bar, 25 μm.
