Figure 8.

Quantification of effects of Rho and caldesmon on focal adhesions visualized by anti-phosphotyrosine staining. The percentage of cells having small, medium, and large focal adhesions was scored. Small refers to dot-like, nonelongated plaques; medium corresponds to typical dash-like adhesions in cultures of SV80 cells in serum-containing medium; and large denotes very large streak-like focal adhesions, typical of Rho-transfected cells. Note that Rho transfection induces formation of large focal adhesions in all cells (compare A and B), whereas caldesmon transfection efficiently decreases the percentage of cells with large focal adhesions, even in cells expressing activated Rho (C and D).