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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Immunity. 2008 Jul 3;29(1):138–149. doi: 10.1016/j.immuni.2008.05.009

Figure 6. Generation of TFH cells is independent of TH17 lineage.

Figure 6

Rag1-/- reconstituted with WT and RORαsg/sg/RORγ KO bone marrow cells (A) or IL-17 KO and IL-17F KO mice and their controls (C) (3 mice per group) were immunized subcutaneously with KLH in CFA. Seven days after the immunization, experimental mice were sacrificed and the TFH cells were determined. Numbers in dot plot quadrants represent the percentages. (B). C57BL/6 mice were immunized with KLH emulsified in CFA with 100 μg of isotype control antibodies or anti-TGFβ blocking Abs (3 mice per group). 7 days later, experimental mice were sacrificed and splenic TFH cells and germinal center B cells were analyzed. Splenocytes were restimulated with KLH for overnight and the production of IL-17 and IFNγ was analyzed in CD4+ gate by intracellular cytokine staining. The results represent one of three individuals with similar results.