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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cytokine. 2008 Jun 12;43(2):114–123. doi: 10.1016/j.cyto.2008.04.015

Fig. 2.

Fig. 2

Ligand binding induces mono-/polyubiquitination of the G-CSFR. ts20 cells expressing V5-tagged WT G-CSFR were incubated at 37°C with G-CSF (100 ng/mL) for the indicated times, lysed, then immunoprecipitated with anti-V5 antibody and blotted with the indicated antibodies. The FK1 antibody (A) recognizes polyubiquitinated proteins whereas the FK2 antibody (B) recognizes both poly- and mono-ubiquitinated proteins. (C) The blot was stripped and re-blotted with anti-V5 antibody. Histograms are shown to the right of each blot depicting the density ratio between bands of FK1 and FK2 to anti-V5, respectively, as a control for receptor protein loading.