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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: Biochemistry. 2007 Jun 20;46(28):8392–8400. doi: 10.1021/bi700289k

Figure 3. Modification of unmodified and acrolein-modified apoE3-NT by fluorescein isothiocyanate.

Figure 3

Unmodified (white bar) and acrolein-modified (grey) apoE3-NT (100 μg protein) were modified with 10-fold molar excess of fluorescein 5(6)-isothiocyanate. The reaction was carried out in 20 mM Tris-HCl, pH 8.0 for 10 min at 37 °C to label accessible lysine residues. Following modification, the samples were dialyzed against TBS to remove excess probe. Fluorescein fluorescence emission intensity was recorded at 517 nm following excitation at 495 nm. Values are average ± SD (n=3). Statistical analysis was carried out by Student's t test (P<0.01).

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