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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: Biochemistry. 2007 Jun 20;46(28):8392–8400. doi: 10.1021/bi700289k

Figure 5. Effect of acrolein modification on tertiary structural features of apoE3-NT.

Figure 5

Panel A. Trp fluorescence emission. Trp fluorescence emission spectra of 35 μg unmodified (solid line, a) or acrolein-modified (broken line, b) apoE3-NT were recorded between 300 and 500 nm following excitation at 295 nm. Panel B. ANS fluorescence emission. Unmodified (solid line, a) or acrolein-modified (broken line, b) apoE3-NT (35 μg protein) were treated with 250 μM ANS in 20 mM sodium phosphate, pH 7.4 for 10 min at 24 °C. The fluorescence emission spectrum of the incubation mixtures was monitored between 400 and 600 nm following excitation at 395 nm. Fluorescence emission spectrum of 250 μM ANS alone in 20 mM sodium phosphate, pH 7.4 (dotted line, c) is shown for comparison.

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