Figure 6. Effect of acrolein modification on the ability of apoE3-NT to bind phospholipid vesicles.

Unmodified (solid line, curve a) or acrolein-modified (dashed line, curve b) apoE3-NT (200 μg) were treated with DMPC MLVs (100 μg DMPC) (∼10:1 lipid:protein molar ratio) in TBS. The mixture was maintained at 24 °C, the transition temperature of DMPC. The change in absorbance at 325 nm was followed as a function of time and shown as normalized absorbance units. In the absence of protein, there was no change in the absorbance of DMPC vesicles (dotted line, curve c). Representative curves from 5 different experiments are shown.