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Unmodified (Panel A) or acrolein-modified (Panel B) apoE3-NT (100 μg) was loaded onto a heparin-Sepharose column in 20 mM sodium phosphate pH 7.4 in a ÄKTA FPLC system. The flow rate was maintained at 0.5 ml/min. A salt gradient consisting of 0-1 M NaCl was applied (black straight line) and the elution of the protein monitored at 280 nm. The black arrow represents the time at which the gradient was initiated.
