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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: Biochemistry. 2007 Jun 20;46(28):8392–8400. doi: 10.1021/bi700289k

Figure 8. Effect of acrolein modification on the LDLr binding ability of apoE3-NT.

Figure 8

DMPC/apoE complexes were incubated with 1 μg of sLDLr/LA3-LA6/Myc in 25 mM Tris-HCl, pH 7.4, 140 mM NaCl, 27 mM KCl, 2 mM CaCl2 for 2 h at 4 °C, followed by IP with anti-c-Myc antibody. LDLr-bound apoE was detected by Western blot using anti-apoE monoclonal antibody 1D7 (top panel). The lane assignments are as follows: lane 1, unmodified apoE3-NT (1 μg); lane 2, acrolein-modified apoE3-NT (1 μg); lane 3, unmodified apoE3-NT (3 μg); lane 4, acrolein-modified apoE3-NT (3 μg); lane 5, Lys143Ala/Lys146Ala/apoE3-NT (3 μg); lane 6, apoE2 (3 μg). A duplicate analysis was carried out in parallel with anti-c-Myc antibody (bottom panel) to confirm the presence of similar levels of LDLr in each reaction mixture.

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