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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: Nature. 2008 Mar 9;452(7187):598–603. doi: 10.1038/nature06716

Figure 3. Translational trajectories and arrests for VE274hp.

Figure 3

Translations were followed on dual-trap optical tweezers35. a, Examples of translation trajectories from four different ribosomes. The five horizontal yellow stripes correspond to the Glu patches (see c). b, Close-up views of trajectories. Data were collected at 2 kHz and smoothed to 10 Hz. c, Translation arrests. The positions (relative to the A-site on the ribosome) where translation arrests occur are indicated by short horizontal bars, blue for the wild-type VE274hp and red for the mutant hairpin VE274hp-G2A. The VE274hp-G2A differs from the wild type only in the first internal Shine-Dalgarno-like sequence (boxed by solid green line).