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. Author manuscript; available in PMC: 2009 Jul 18.
Published in final edited form as: J Mol Biol. 2008 May 17;380(4):623–635. doi: 10.1016/j.jmb.2008.05.025

Figure 5.

Figure 5

Percentage tCA bound to the A. variabilis PAL double mutant as a function of L-Phe loaded. The indicated concentration of Phe was incubated in the presence of 13 µM A. variabilis PAL double mutant for a sufficient period of time to convert all substrate to product. Samples were then desalted to remove excess L-Phe and unbound tCA and injected on a C4-RP column for analysis. Results appear to show that two PAL active sites remain occupied with the product tCA which is not easily dislodged.