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Close-up view of active site residues that have direct interaction with the MIO and modeled tCA in the A. variabilis PAL double mutant monomer B, the only monomer that has clear electron density for the phenyl ring of tCA. Extra electron density are also observed in the other monomers, but none could be modeled as a ring. The electron density map (2Fo-Fc) is contoured at 1σ level
