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. Author manuscript; available in PMC: 2009 Jul 18.
Published in final edited form as: J Mol Biol. 2008 May 17;380(4):623–635. doi: 10.1016/j.jmb.2008.05.025

Table 1.

Comparison of activity and kinetic constants of wild-type R. toruloides PAL, wild-type A. variabilis PAL and A. variabilis PAL double mutant

Specific activity (IU/mg) Km (mM) kcat (s−1) kcat/Km (mM−1s−1)
wt R. toruloides PAL 4.5 1.1 16 15
wt A. variabilis PAL 7 1.7 0.06 4.6 77
A. variabilis PAL double mutant 2.2 0.05 4.0 80