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. Author manuscript; available in PMC: 2008 Sep 30.
Published in final edited form as: J Biol Chem. 2003 Dec 15;279(10):9475–9480. doi: 10.1074/jbc.M307246200

FIG. 4. Discovery of an ovalicin-sensitive human methionine aminopeptidase-1 allele.

FIG. 4

Wild type (WT) and T334A human MetAP-1 alleles were expressed in ΔMAP1 yeast, and a dot titration was subsequently performed. Shown is substitution of threonine 334 with alanine renders human MetAP-1 (hMetAP-1) sensitive to ovalicin (A), fumagillin (B), and TNP-470 (C). Plates were photographed after a 2-day incubation at 30 °C.