Figure 2.

Testosterone-induced Ca²⁺ mobilization in IC-21 cells. (A) Testosterone causes an immediate Ca²⁺ spike. (B) Cells were treated with cyproterone for 30 min or flutamide for 60 min before adding testosterone. (C) Cells were incubated with EGTA for 90 s before addition of testosterone. (D) Cells were treated with the phospholipase C inhibitor U-73122 or with the inactive control compound U-73343 for 2 min before adding testosterone. (E) Cells were pretreated with pertussis toxin for 16 h (+ PTX) before addition of testosterone. (F) Cells were incubated with Mn²⁺ for 2 min before adding testosterone. Arrows indicate addition of substances to IC-21 cells.