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Surface binding sites of testosterone and their internalization in intact IC-21 cells. (A) Cells were incubated with testosterone-BSA-FITC for various periods between 5 s and 1 h and then analyzed by flow cytometry. (B) CLSM of cells incubated with testosterone-BSA-FITC for 5 s and 1 h. Optical slices of 0.5 μm. Bars, 10 μm.
