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. 1999 Oct;10(10):3113–3123. doi: 10.1091/mbc.10.10.3113

Figure 8.

Figure 8

CLSM colocalization of the sequestered surface binding sites of testosterone. (A) IC-21 cells were incubated in parallel with testosterone-BSA-FITC and LysoTracker Red DND-99 at 37°C for 1 h. Testosterone-BSA-FITC did not colocalize with acidic vesicles stained with LysoTracker Red DND-99. (B) Testosterone-BSA-FITC was not sequestrated within clathrin-coated vesicles as detected by anti-clathrin antibodies and the Cy3-labeled corresponding secondary antibody. (C) Internalized punctate fluorescence of testosterone-BSA-FITC was not labeled with an anti-caveolin antibody and its corresponding secondary TRITC-conjugated antibody. Bars, 10 μm.