Figure 9.
Flow cytometry of the internalization of surface-bound testosterone-BSA-FITC in IC-21 cells. (A) Cells were incubated for 15 min with testosterone-BSA-FITC (10−6 M) in the absence (Control) or in the presence of a 10-fold excess of unlabeled testosterone (+ T) or 1-dehydrotestosterone (+ 1-DeHT). Values normalized to controls are given as means ± SD from five different experiments. (B) Cells were equilibrated for 30 min at the indicated temperatures and then incubated with 1.5 × 10−5 M testosterone-BSA-FITC for 1 or 15 min at the same temperatures. Values represent means ± SD from at least two different experiments.